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Urinalysis | Urine Analysis

Urine routine tests for urine analysis are grouped in three parts; physical, chemical and microscopic examinations also known as Urinalysis.

Urine routine tests for urine analysis are grouped in three parts; physical, chemical, and microscopic examinations also known as Urinalysis.

Urinalysis  |  Urine Analysis

Urinalysis is the test performed in a clinical pathology lab in a hospital or lab where urinalysis means the analysis of urine and also known as urine examination, this includes physical, biochemical, microscopic examination, and culture (if needed).

Urinalysis | Urine Analysis
Urinalysis | Urine Analysis


Urinalysis (URINE ANALYSIS) Will Indicate:-


(i) The state of the kidneys and the urinary tract-


Urinalysis Chemical tests for the presence of protein or blood; together with physical properties and microscopic examination for casts, cells, and certain crystals are very helpful in assessing and treating renal and urinary tract disease. 


(ii) Information about metabolic and systemic abnormalities


Tests for glucose, ketone bodies, bilirubin, and urobilinogen are useful parameters in the diagnosis of metabolic and systemic disorders such as diabetes and jaundice in Urinalysis 



Composition of Urine:-


Urine is the fluid containing water-soluble waste products excreted from the blood via the kidney Urine is mainly composed of 95% water and the rest is made up of urea, uric acid, creatinine sodium, potassium chloride, calcium, phosphate, etc. 

The composition of urine varies a great d and is affected by three factors:

  • Nutritional status of the individual
  • State of metabolic processes
  • The ability of the kidneys to selectively handle the material presented to them.


Properties of normal urine :


Properties of normal urine  (Normal urine test results chart):–

Volume per day

 

Neonates :

30-60 ml

Children :

 400-1400ml

Adult :

600_2500 ml

Ph:

4.5-8.0(mean:6.1)

Organic constituents 24 hours

Urea

:15-30gm

Creatine :

1.0-1.8g

Uric acid

:0.3-o.6g

Protein(albumin)

:-0-0.1g

Glucose

<0.5g

Dry weight.

:55-70g/hours

  
  
  
  


Collection and Preservation of Urine:-


Urine samples for Urinalysis should be collected in a clean, dry container and examined as soon as possible. In most cases, the first specimen freely voided in the morning is preferred although a specimen collected 2 to 3 hours after eating is suitable when testing for glucose. 

  • On standing, red blood cells and white blood cells are destroyed; casts are decomposed; glucose is lost and bacterial contamination of urine takes place causing alkalization of the sample. 
  • Therefore, the specimen must be examined when fresh, ideally within 30 minutes of collection


ROUTINE TESTS FOR URINE ANALYSIS:-


The routine tests for urine analysis are grouped in three parts; physical, chemical, and microscopic examinations. The below table shows the tests routinely performed under each section. Certain tests are graded according to their degree of accuracy: screening or qualitative tests; semi-quantitative. tests; and quantitative tests. 

Urinalysis | Urine Analysis
Urinalysis | Urine Analysis


The results of qualitative tests are reported as positive or negative; or, present or absent. For semi-quantitative tests, results are graded as negative, trace, 1+, 2+, 3+, or 4+, or sometimes results are given as small, moderate, or large quantities. The quantitative tests, on the other hand, determine accurately the amount of substance that they detect in urine


TESTS ROUTINELY PERFORMED FOR URINALYSIS (URINE ANALYSIS)


TESTS ROUTINELY  PERFORMED FOR URINALYSIS

1.PHYSICAL EXAMINATION

NAMES OF METHOD

 

COLOUR

Physical view

TRANSPARENCY

Physical view

ODOUR

 

PH

 ph meter

SPECIFIC GRAVITY

(URINE METER)

  
  

2.CHEMICAL EXAMINATION 

 

1.GLUCOSE TEST

 

2.PROTINE TEST


3.ALBUMIN TESt

 

4.KETONES TEST

 

5.BILIRUBIN TEST

 

6.UROBILOGEN TEST

 

7.OCCULT BLOOD

 
  
  
  

MICROSCOPIC EXAMINATION OF URINARY SEDIMENT

1.RED BLOOD CELLS

 

2.WHITE BLOOD CELLS

 

3. EPHITHILIAL CELLS

 

4.CASTS

 

5.BACTERIA AND OTHER MICROORGANISMS

 

6.CRYSTALS

 

OTHER COMPONENTS

 



Types of Test for Urine Examination 


  1. PHYSICAL EXAMINATION - click here
  2. CHEMICAL EXAMINATION  - click here
  3. MICROSCOPIC EXAMINATION 


Summary of Urine Analysis



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